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Ironic ultranationalistic memes about Western European countries (Netherlands, Luxembourg, Germany, France, Italy, Spain, Portugal, Greece, UK, Ireland, Denmark (incl ...
α-tubulin和β-tubulin实际检测条带均在55kDa左右。 作为内参,β-tubulin的蛋白水平通常不会发生改变,因此被广泛用于Western Blotting时上样量是否一致的参照,也常被用于免疫染色观察细 …
20 thg 10, 2020 · 13 votes, 27 comments. truehealth science and medical science are 2 different programs! health sci at western is more like health studies while medical science is actual …
Western Blotting先使用PAGE(聚丙烯酰胺凝胶电泳)电泳分离出待检测的蛋白质,再用电场装置印迹至固相介质(如PVDF膜)上,固相介质以非共价键形式吸附蛋白质,且能保持电泳分离 …
PLEASE READ THE RULES BEFORE POSTING Welcome to the Reddit hub for all types of cowboy boots, from current western boots to handmade exotics, vintage, and custom styles, …
western blot 裁膜问题? 在其他经验贴查到很多杂志要求整张膜敷抗体的图像,那就意味着western blot转膜结束后要敷目的显影,而后用抗体洗脱液,再敷内参显影。
膜上的蛋白是指样品蛋白还是吸附在膜上的 一抗 、二抗蛋白呢? 如果是样品蛋白,除了过度转膜导致其直接穿过膜这种情况之外,实验条件下应该不会让蛋白脱离膜,除了洗膜液。 而一抗二 …
为什么western blot 的条带有很多非特异性条带? 有可能是一抗的原因吗? 最近刚开始跑目的蛋白的条带,结果发现有很多非特异性条带。 我的样品是从细胞里提取的蛋白,在冰上裂 …
Western blot(蛋白质印迹法)是一种用于检测和分析特定蛋白质的实验技术,广泛应用于生物医学研究中。以下是Western blot的详细原理、实验步骤、数据处理和作图过程。 一、实验原理 …
I don't really want to be 'sold' on the idea of an online university over B&M. Most universities are trying to offer online degrees for working adults (especially Master's programs). But, let's be …
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